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coating buffer (Agdia Inc)

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Agdia Inc coating buffer
Coating Buffer, supplied by Agdia Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/coating+buffer/buffer+coating/pmc13149247-299-6-10
Average 86 stars, based on 1 article reviews

coating buffer - by Bioz Stars, 2026-10

86/100 stars

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Article Title: Breeding Tm-1 -based tomato rootstocks, resistant to tomato brown rugose fruit virus, to impede soil-mediated viral infections
Article Snippet: .. Sampled leaves were ground in a coating buffer (according to Agdia protocol, Agdia, Elkhart, IN, USA) (~0.6 g/ml) and then incubated for 3 h at 37 °C for adhesion. .. Following x4 washes with PBS-Tween (0.05% Tween-20 in PBS), ToBRFV-specific antisera diluted 1:3000 in PBS-2% milk were added and incubated at 37 °C for 2 h. Following washes (x4) with PBS-Tween, samples were incubated with alkaline phosphatase (AP) -conjugated goat anti-rabbit (IgG) (Sigma, Steinheim, Germany) diluted 1:4000 in PBS-2% milk for 3 h at 37 °C.

Article Title: Contribution of the tobamovirus resistance gene Tm-1 to control of tomato brown rugose fruit virus (ToBRFV) resistance in tomato.
Article Snippet: .. Samples were collected 30 DPI, ground in a coating buffer (Agdia), and incubated for 3 h at 37°C with a 1:5000 dilution of the anti-ToBRFV antiserum. .. For the subsequent detection step, samples were treated with alkaline phosphatase-conjugated goat anti-rabbit IgG (Sigma, Steinheim, Germany) for 3 additional hours at 37°C.

Article Title: Identification and Mapping of Tomato Genome Loci Controlling Tolerance and Resistance to Tomato Brown Rugose Fruit Virus
Article Snippet: .. Samples were taken 30 days after inoculation, ground in coating buffer (Agdia) and incubated for 3 h at 37 °C with 1:5000 dilution of the specific laboratory-produced antiserum (anti-ToBRFV or anti-ToMV). .. Detection was carried out by incubating the samples with AP-conjugated goat anti-rabbit (IgG) (Sigma, Steinheim, Germany) for 3 h at 37 °C.

Article Title: Contribution of the Tobamovirus resistance gene Tm-1 to control of ToBRFV resistance in tomato
Article Snippet: .. Samples were collected 30 DPI, ground in a coating buffer (Agdia), and incubated for 3 h at 37°C with a 1:5000 dilution of the anti-ToBRFV antiserum. .. For the subsequent detection step, samples were treated with alkaline phosphatase-conjugated goat anti-rabbit IgG (Sigma, Steinheim, Germany) for 3 additional hours at 37°C.

Article Title: Pepper Plants Harboring L Resistance Alleles Showed Tolerance toward Manifestations of Tomato Brown Rugose Fruit Virus Disease
Article Snippet: .. Samples were ground in coating buffer (Agdia) at a ratio of ~600 μL/μg sample and incubated with 1:5000 dilution of specific antibodies against ToBRFV [ ] in PBS for 3 h at 37 °C. .. For detection, alkaline phosphatase (AP)-conjugated goat antirabbit IgG (Sigma, Steinheim, Germany, 1:5000 in PBS) and p-nitro phenyl phosphate substrate (Sigma, 0.6 mg/mL) were used.

Article Title: Tissue-specific resistance and susceptibility to the tomato brown rugose fruit virus (ToBRFV) conferred by Solanum pennellii loci.
Article Snippet: .. Leaves were ground in a coating buffer (according to Agdia protocol), and following adhesion to a microtiter plate, the plate was incubated for three h, at 37 °C, with ToBRFV-specific antiserum (1:3000 dilution) [6]. .. For detection, the samples were incubated with alkaline phosphatase (AP)-conjugated goat antirabbit (IgG) (Sigma, Steinheim, Germany) for three h, at 37 °C, after which 0.6 mg/mL P-nitro phenyl phosphate (Sigma) coloring substrate was added to each well.

Article Title: Tissue-specific resistance and susceptibility to the tomato brown rugose fruit virus (ToBRFV) conferred by Solanum pennellii loci
Article Snippet: .. Leaves were ground in a coating buffer (according to Agdia protocol), and following adhesion to a microtiter plate, the plate was incubated for three h, at 37 °C, with ToBRFV-specific antiserum (1:3000 dilution) [ ]. .. For detection, the samples were incubated with alkaline phosphatase (AP)-conjugated goat anti-rabbit (IgG) (Sigma, Steinheim, Germany) for three h, at 37 °C, after which 0.6 mg/mL P-nitro phenyl phosphate (Sigma) coloring substrate was added to each well.

Article Title: Synthesis and Characterization of Durable Antibiofilm and Antiviral Silane-Phosphonium Thin Coatings for Medical and Agricultural Applications
Article Snippet: Indirect ELISAs (Thermo Fisher Multiskan FC) were performed on leaves that had ToBRFV infection symptoms. .. Samples were placed in a coating buffer (Agdia) and incubated for 3 h at 37 °C with a 1:5000 dilution of the ToBRFV antiserum. .. Detection was done by incubating the samples with AP-conjugated goat antirabbit IgG (Sigma, Germany) for 3 h at 37 °C.

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